doi:

DOI: 10.3724/SP.J.1249.2017.02117

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:2 PP.117-121

Expression and immunological identification of MBP-Bmlp7 from Bombyx mori


Abstract:
The open reading frame of Bmlp7 from silkworm (Bombyx mori) was chemically synthesized and cloned into plasmid pMAL-c5e. The recombinant plasmid pMAL-Bmlp7 was transformed into E. coli BL21 and expressed as a fusion protein. The protein was purified using amylose affinity chromatography and purified to homogeneity. The immunoglobulins E(IgE) reactivity of the fusion protein was assayed by western blotting using sera of patient with silkworm allergies. The results show that the Bmlp7 fusion protein has IgE reactivity with the patient sera.

Key words:immunology,Bombyx mori,30 kD lipoprotein,expression and purification,immunoblotting,fusion protein,immnogenicity

ReleaseDate:2017-04-10 18:10:19



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