doi:

DOI: 10.3724/SP.J.1249.2017.03272

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:3 PP.272-277

Three-dimensional spatial resolution calibration of the coherent anti-Stokes Raman scattering microscopy


Abstract:
The spatial resolution is one of the most important indices for measuring the performance of coherent anti-stokes Raman scattering (CARS) microscopy system. Accordingly, we acquire the three dimensional images of the polystyrene micro-balls with 110 nm diameter in order to reconstruct the point spread function of the system on x-y and x-z planes based on the two dimensional array consisting of position data of each point and spectral data. The results show that lateral and axial spatial resolutions of the homemade CARS microscopy are about 484 nm and 3.17 μm, respectively.

Key words:optical engineering,coherent anti-Stokes Raman scattering microscopy,imaging reconstruction,point spread function,resolution,nonlinear optics,Raman scattering,information optics

ReleaseDate:2017-06-16 14:08:44



[1] 刘立新,屈军乐,林子扬,等. 荧光寿命成像及其在生物医学中的应用[J].深圳大学学报理工版,2005,22(2):133-141. Liu Lixin, Qu Junle, Lin Ziyang, et al. Fluorescence lifetime imaging and its biomedical applications[J]. Journal of Shenzhen University Science and Engineering, 2005, 22(2): 133-141.(in Chinese)

[2] 邵永红,李 恒,王 岩,等.基于同步扫描相机的荧光寿命测量系统研究[J].深圳大学学报理工版,2009,26(4):331-336. Shao Yonghong, Li Heng, Wang Yan, et al. A fluorescence lifetime spectrometer based on a synchroscan streak camera[J]. Journal of Shenzhen University Science and Engineering, 2009, 26(4): 331-336.(in Chinese)

[3] 牛憨笨,陈丹妮,尹 君.细胞内分子检测及成像技术研究[J].深圳大学学报理工版,2011,28(1):1-16. Niu Hanben, Chen Danni, Yin Jun. Advances in approaches of molecules detecting and imaging in cells[J]. Journal of Shenzhen University Science and Engineering, 2011, 28(1): 1-16.(in Chinese)

[4] Lichtman J W, Conchello J A. Fluorescence microscopy[J]. Nature Methods, 2005, 2(12): 910-919.

[5] Mukamel S. Principles of nonlinear optical spectroscopy[M]. New York, USA: Oxford University Press, 1999.

[6] Jalbert I, Stapleton F, Papas E, et al. In vivo confocal microscopy of the human cornea[J]. British Journal of Ophthalmology, 2003, 87(2): 225-236.

[7] Kiesslich R, Burg J, Vieth M, et al. Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo[J]. Gastroenterology, 2004, 127(3): 706-713.

[8] Freudiger C W, Min W, Saar B G, et al. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy[J]. Science, 2008, 322(5909): 1857-1861.

[9] Wang Hui, Sun Yubing, Yi Jinhui, et al. Fluorescent porous carbon nanocapsules for two-photon imaging, NIR/pH dual-responsive drug carrier, and photothermal therapy[J]. Biomaterials, 2015, 53: 117-126.

[10] Karuna A, Masia F, Borri P, et al. Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard[J]. Journal of Raman Spectroscopy, 2016, 47(9): 1167-1173.

[11] Lee Y J, Vega S L, Patel P J, et al. Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy[J]. Tissue Engineering Part C: Methods, 2014, 20(7): 562-569.

[12] Krafft C, Dietzek B, Schmitt M, et al. Raman and coherent anti-Stokes Raman scattering microspectroscopy for biomedical applications[J]. Journal of Biomedical Optics, 2012, 17(4): 040801.

[13] Goodhead R M, Moger J, Galloway T S, et al. Tracing engineered nanomaterials in biological tissues using coherent anti-Stokes Raman scattering (CARS) micro-scopy: a critical review[J]. Nanotoxicology, 2015, 9(7): 928-939.

[14] Slipchenko M N, Cheng Jixin. Nonlinear Raman spectroscopy: coherent anti-Stokes Raman scattering (CARS)[M]//Encyclopedia of Biophysics. Berlin: Springer Berlin Heidelberg.,2013: 1744-1750.

[15] Maker P D, Terhune R W. Study of optical effects due to an induced polarization third order in the electric field strength[J]. Physical Review, 1965, 137(3A): A801-A818.

[16] Duncan M D, Reintjes J, Manuccia T J. Scanning coherent anti-Stokes Raman microscope[J]. Optics Letters, 1982, 7(8): 350-352.

[17] Zumbusch A, Holtom G R, Xie X S. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering[J]. Physical Review Letters, 1999, 82(20): 4142-4145.

[18] Cheng Jixin, Volkmer A, Xie X S. Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy[J]. Journal of the Optical Society of America B, 2002, 19(6): 1363-1375.

[19] Cheng Jixin, Jia Y K, Zheng Gengfeng, et al. Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology[J]. Biophysical Journal, 2002, 83(1): 502-509.

[20] Hellerer T, Axäng C, Brackmann C, et al. Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy[J]. Proceedings of the National Academy of Sciences, 2007, 104(37): 14658-14663.

[21] Moura C C, Tare R S, Oreffo R O C, et al. Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration[J]. Journal of The Royal Society Interface, 2016, 13(118): 20160182.

[22] Ganikhanov F, Evans C L, Saar B G, et al. High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy[J]. Optics Letters, 2006, 31(12): 1872-1874.

[23] Evans C L, Potma E O, Xie X S. Coherent anti-Stokes Raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility χ(3) for vibrational microscopy[J]. Optics Letters, 2004, 29(24): 2923-2925.

[24] Breunig H G, Weinigel M, Bückle R, et al. Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber[J]. Laser Physics Letters, 2013, 10(2): 025604.

[25] Chemnitz M, Baumgartl M, Meyer T, et al. Widely tuneable fiber optical parametric amplifier for coherent anti-Stokes Raman scattering microscopy[J]. Optics Express, 2012, 20(24): 26583-26595.

[26] Zumbusch A, Holtom G R, Xie X S. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering[J]. Physical Review Letters, 1999, 82(20): 4142.

[27] Nan X, Cheng J X, Xie X S. Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy[J]. Journal of Lipid Research, 2003, 44(11): 2202-2208.