DOI: 10.3724/SP.J.1249.2017.05457

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:5 PP.457-463

Characteristics of antioxidant activity of soybean PM1 protein and enhancement of tolerance of recombinant yeast to copper stress

Cu2+ is an essential micronutrient for plant growth, but it is toxic when plant growth under excess copper stress. Soybean GmPM1 protein belongs to late embryogenesis abundant (LEA) group 4 (LEA4) proteins, which has a high proportion of histidine residues in the protein sequence. Firstly, we investigate the protective function and mechanisms of GmPM1 protein in plant under Cu2+ stress. The leaves of soybean seedling are withered under 150 μmol/L CuSO4 stress, and at the meantime the expression of GmPM1 gene in the young leaves was up-regulated in 3 h and 24 h of the stress. Secondly, the yeast expression plasmid of pYES2-GmPM1 is constructed and then transformed into the copper-sensitive yeast mutant ΔYAP1 to create recombinants of ΔYAP1-GmPM1. The recombinant yeast expressing GmPM1 protein could enhance the tolerance to Cu2+ stress. Then, the activities of scavenging hydroxyl radicals of GmPM1 and GmPM1-C protein in vitro are determined by using Cu-ascorbic acid system, which is rich in histidine residual in their sequence. The results show that GmPM1 could chelate Cu2+ through histidine residual in the C-terminal of GmPM1 protein and exert the activity of scavenge hydroxyl radicals, thus could improve the tolerance of plants to Cu2+ stress.

Key words:soybean,GmPM1 protein,histidine,Cu2+ stress,scavenging hydroxyl radicals,recombinant yeast

ReleaseDate:2017-10-20 02:07:29

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