DOI: 10.3724/SP.J.1249.2017.05526

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:5 PP.526-531

Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging

Multi-particle tracking is one of the most challenging aspects of living cell imaging. We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm, respectively. The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time. Therefore, this system is a very useful tool for studying the intermolecular interaction in living cell.

Key words:optical engineering,three-dimensional imaging,multi-particle,point spread function,nano-posi-tioning,living cell,dynamic tracking

ReleaseDate:2017-10-20 02:07:32

[1] Xie X S, Yu J, Yang W Y. Living cells as test tubes[J]. Science, 2006,312(5771):228-230.

[2] 牛憨笨,陈丹妮,尹君.细胞内分子检测及成像技术研究[J].深圳大学学报理工版,2011,28(1):1-16. Niu Hanben,Chen Danni, Yin Jun.Advances in approaches of molecules detecting and imaging in cells[J].Journal of Shenzhen University Science and Engineering,2011,28(1):1-16.(in Chinese)

[3] 许改霞,杨坚泰,Roy Indrajit,等.纳米颗粒在穿透血脑屏障研究中的应用[J].深圳大学学报理工版,2009,26(1):1-8. Xu Gaixia,Yang Kentye,Roy Indrajit,et al.The application of nanoparticle in BBB transmigration[J].Journal of Shenzhen University Science and Engineering,2009,26(1):1-8.(in Chinese)

[4] Ober R J, Ram S, Ward E S. Localization accuracy in single-molecule microscopy[J]. Biophysical Journal, 2004,86(2):1185-1200.

[5] Hess S T, Girirajan T P K, Mason M D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy[J]. Biophysical Journal, 2006,91(11):4258-4272.

[6] Rust M J, Bates M, Zhuang X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)[J]. Nature Methods, 2006,3(10):793-796.

[7] 邵永红,李恒,王岩,等.基于同步扫描相机的荧光寿命测量系统研究[J].深圳大学学报理工版,2009,26(4):331-336. Shao Yonghong, Li Heng,Wang Yan,et al.A fluorescence lifetime spectrometer based on a synchroscan streak camera[J].Journal of Shenzhen University Science and Engineering,2009, 26(4):331-336.(in Chinese)

[8] Vo-Dinh T, Masters B R. Biomedical photonics handbook[J]. Journal of Biomedical Optics, 2004,9(5):1110-1111.

[9] Mizushima N, Levine B. Autophagy in mammalian development and differentiation[J]. Nature Cell Biology, 2010,12(9):823-830.

[10] 陈丹妮,于斌,刘夏,等.基于数字微镜装置的结构光照明层析成像[J].深圳大学学报理工版,2008,25(4):376-380. Chen Danni,Yu Bin,Liu Xia,et al.Sectioned imaging of DMD-based structured illumination fluorescence microscopy[J].Journal of Shenzhen University Science and Engineering,2008,25(4):376-380.(in Chinese)

[11] Huang B, Wang W, Bates M, et al. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy[J]. Science, 2008,319(5864):810-813.

[12] Juette M F, Gould T J, Lessard M D, et al. Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples[J]. Nature Methods, 2008,5(6):527-529.

[13] Pavani S R P, Piestun R, Thompson M A, et al. Three-dimensional super-resolution imaging with a double-helix microscope[C]//Proceedings Advances in Imaging. Vancouver,Canada:OSA Publishing, 2009.

[14] Herbert S, Soares H, Zimmer C, et al. Single-molecule localization super-Resolution microscopy:deeper and faster[J]. Microscopy and Microanalysis, 2012,18(6):1419-1429.

[15] Ram S, Kim D, Ober R J, et al. 3D single molecule tracking with multifocal plane microscopy reveals rapid intercellular transferrin transport at epithelial cell barriers[J]. Biophysical Journal, 2012,103(7):1594-1603.

[16] Abrahamsson S, Chen J, Hajj B, et al. Fast multicolor 3D imaging using aberration-corrected multifocus microscopy[J]. Nature Methods, 2013,10(1):60-63.

[17] Chen Danni, Yu Bin, Li Heng, et al. Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution[J]. Optics Letters, 2013,38(19):3712-3715.

[18] 王炯, 黄维琳, 汪诚, 等. 巨噬细胞吞噬凋亡中性粒细胞及吞噬诱导巨噬细胞死亡的实时动态观察[J]. 中国科学:生命科学, 2014, 44(6):571-577. Wang Jiong, Huang Weilin, Wang Cheng, et al. Dynamic process of phagocytosis and fates of macrophages after their ingestion of apoptotic neutrophils[J]. Scientia Sinica Vitae, 2014,44(6):571-577.(in Chinese)

[19] Schechner Y Y, Piestun R, Shamir J. Wave propagation with rotating intensity distributions[J]. Physical Review E, 1996,54(1):50-53.

[20] Piestun R, Schechner Y Y, Shamir J. Propagation-invariant wave fields with finite energy[J]. JOSA A, 2000,17(2):294-303.

[21] 李恒, 于斌, 陈丹妮, 等. 高效双螺旋点扩展函数相位片的设计与实验研究[J]. 物理学报, 2013,62(12):124201. Li Heng, Yu Bin, Chen Danni,et al. Design and experimental demonstration of high-efficiency double-helix point spread function phase plate[J].Acta Physica Sinica, 2013,62(12):124201.(in Chinese)