doi:

DOI: 10.3724/SP.J.1249.2017.05526

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:5 PP.526-531

Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging


Abstract:
Multi-particle tracking is one of the most challenging aspects of living cell imaging. We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm, respectively. The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time. Therefore, this system is a very useful tool for studying the intermolecular interaction in living cell.

Key words:optical engineering,three-dimensional imaging,multi-particle,point spread function,nano-posi-tioning,living cell,dynamic tracking

ReleaseDate:2017-10-20 02:07:32



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