DOI: 10.3724/SP.J.1249.2018.01001

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:1 PP.1-7

Target knockout of ck2α gene and its functional analysis

Protein kinase CK2α was knocked out in human colorectal cancer cell line HCT116 by using CRISPR-Cas9 gene editing technique to investigate CK2α's function. PX459-g CK2α vector was successfully transfected into HCT116 cells, and screened gene knockout cell line to further analysis whether it was involved in the proliferation and migration of cancer cells. The results show that HCT116 cell line with ck2α knockout is successfully obtained by CRISPR-Cas9 gene editing technique. ck2α knockout can significantly reduce the cell proliferation rate and migration ability, and accelerate the process of cellular senescence.

Key words:gene editing,CRISPR-Cas9,gene knockout,protein kinase CK2,cell proliferation,colorectal cancer,cellular senescence

ReleaseDate:2018-03-20 15:26:54

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