DOI: 10.3724/SP.J.1249.2018.01008

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:1 PP.8-14

JAG's regulation in controlling the chlorophyll a/chlorophyll b ratio in Arabidopsis floral organ

JAGGED (JAG), a C2H2 zinc finger transcription factor, is a key regulator of cell division and differentiation in Arabidopsis. Studies on dissecting its complex downstream regulatory network have attracted plant biologists for many years. To further dissect the role of JAG and analyze the downstream regulation network, we investigate the floral phenotypes of transgenic plants overexpressing an inducible form of JAG and observe the strong morphological defects in flower development when JAG is induced. Furthermore, we also detect a significant change of the chlorophyll a/b ratio in the JAG-overexpressing floral tissues. Subsequent gene expression assay shows that this phenotype is likely attributable to the increased level of CAO, a key factor involved in chlorophyll a to b transition. In the JAG overexpression plant, this regulation of CAO by JAG appears to be indirect. In addition, we also analyze the published micro-array and ChIPseq data that have been used for identifying the target genes of JAG, and reveal that 23 JAG direct targets are associated with chloroplast development. These results suggest the potential important role of JAG in controlling chlorophyll biogenesis and will hopefully provide new insights in understanding the regulatory mechanisms of JAG in plant organ development and function.

Key words:plant physiology,chloroplast,chlorophyll,Arabidopsis thaliana,JAGGED,transcriptional factor,network

ReleaseDate:2018-03-20 15:26:54

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