DOI: 10.3724/SP.J.1249.2018.01015

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:1 PP.15-21

The effect of RNA interference of MHR1 on cellulase activity in Myceliophthora thermophila

The potential negative regulatory factor MHR1 was screened by RNA-Seq technique. A positive transformant MtR5 was successfully obtained by constructing the RNAi expression vector for mhr1 through the designing of the RNA interference sequence aimed at mhr1 and the use of the strong promoter of pdc gene. Real-time quantitative PCR, enzymatic and protein concentration determination show that the cellulase activities, extracellular protein concentration, and main cellulase genes expression of the transformant MtR5 are higher than those of the wild type under both inducing and non-inducing conditions. The above results elucidate the relationship between MHR1 and cellulase gene regulation for the first time and the association between the protein MHR1 and carbon catabolite repression. MHR1 works as a novel transcription factor associated with the cellulase gene expression and it is a repressor in carbon catabolite repression.

Key words:thermophilic fungi,Myceliophthora thermophila,RNA interference,cellulase,MHR1 factor,cellulose biomass

ReleaseDate:2018-03-20 15:26:54

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