DOI: 10.3724/SP.J.1249.2018.02166

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:2 PP.166-171

Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.

Aiming to find bioactive natural products with novel structures from the secondary metabolites of actinomycetes and through screening the in vitro antibacterial activity, we find that the fermentation broth of the soil-derived Streptomyces sp. shows inhibitory activity against several bacteria. The strain is then fermented in a large scale. Eleven compounds (1-11) from the ethyl acetate extract are isolated by using various column chromatography. Their structures are (E)-3-(methylthio)acrylic acid (1), (E)-3-(methylthio)acrylamide (2), (E)-cinnamic acid (3), (E)-4-phenyl-3-butenoic acid (4), 3-hydroxy-4-methoxycinnamamide (5), 3-indoleketol (6), N-acetyl-β-oxotryptamine (7), 2-acetylemodin (8), 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (9), cyclo (Phe-Pro) (10), and (3S-cis)-hexahydro-3-[(3,4-dihydroxyphenyl)methyl]pyrrolo[1,2-a]pyrazine-1,4-dione (11), which are identified by HRESIMS, 1D and 2D NMR analysis, and comparison of their NMR data with those published previously. Compounds 4-6, 8, 9 and 11 are firstly discovered from Streptomyces and the 13C NMR data of compound 2 is also firstly reported. Compounds 2-4, 6, 8 and 9 are assessed for in vitro antibacterial activity. Results show that the compounds 3 and 6 display weak activity against the gram-positive Bacillus subtilis and Mycobacterium smegmatis, while compound 8 exhibits weak activity against Bacillus subtilis and Mycobacterium smegmatis and Staphylococcus aureus.

Key words:bioactive natural products,actinobacteria,Streptomyces sp.,column chromatography,secondary metabolites,antibacterial activity

ReleaseDate:2018-03-20 15:26:57

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