DOI: 10.3724/SP.J.1249.2018.02172

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:2 PP.172-178

Bioinformatical analysis of genes encoding APC/C complex and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii

Chlamydomanos reinhardtii is a model microalgae for lipids production. There is a contradiction between lipids biosynthesis and biomass accumulation in C. reinhardtii. Revealing regulation mechanisms of cell cycle can prove a new direction for promoting biomass in C. reinhardtii. In this paper, the gene structures, protein characteristics, and phylogenetic tree of anaphase-promoting complex/cyclosome (APC/C) gene family involving in cell cycle control are analyzed through bioinformatics. Totally 13 genes of APC/C family are identified in C. reinhardtii, and one gene cluster is found in chromosome 13. The phylogenetic tree shows that APC/C proteios, except the activation factors CrCDC20 and CrCDH1 from C. reinhardtii, have low homologous with the proteios in higher plants. Meanwhile, the gene expression profiles of CrCDC20 under sulfur or nitrogen deficiency conditions are determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction (rt-RT-qPCR). The results show that CrCDC20 is up-regulate under sulfur deficiency but down-regulate under nitrogen deficiency, which indicates that CrCDC20 might involve in nutrition deficiency stresses.

Key words:molecular biology of microalgae,biodiesel,cell cycle,E3 ubiquitin ligase,phylogenetic analysis,real-time fluorescence quantitative reverse transcription polymerase chain reaction (rt-RT-qPCR)

ReleaseDate:2018-03-20 15:26:57

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