doi:

DOI: 10.3724/SP.J.1142.2013.60603

Plant Science Journal (植物科学学报) 2013/31:6 PP.603-608

Techniques and Methods for the Practical Use of Xylem Pressure Probes


Abstract:
Xylem pressure probes are the only technique used currently to measure plant xylem pressure directly.This technique includes devices for accurate positioning, pressure probe systems and signal acquisition.Negative pressure of the xylem vessels is transmitted to the pressure sensor by conduction medium when the capillary probe punctures the xylem vessels, with the pressure then detected and transmitted to the data acquisition system.In this paper, the use and precautions of xylem pressure probes are described in detail, including the preparation of glass capillary probes, degassed water, pressure calibration, installation, and measurement of pressure probes.

Key words:Xylem,Pressure probe,Structure and composition,Measurement methods

ReleaseDate:2015-04-15 13:22:58



[1] Bahadur R,Tripathi R P.Sampling procedure for measurement of xylem-water potential by pressure-chamber technique[J].Indian J Agric Sci, 1995, 65(11):803-831.

[2] 王军,孟祥增.热电偶水势测定仪[J].传感器技术, 1997, 16(5):41-45.

[3] 荆家海,肖庆德.利用热电偶湿度计测定水势[J].植物生理学通讯, 1986(1):51-53.

[4] Martinez E M,Cancela J J,Cuesta T S,Neira X X.Review.Use of psychrometers in field measurements of plant material:accuracy and handling difficulties[J].Span J Agric Res, 2011, 9(1):313-328.

[5] Balling A,Zimmermann U.Comparative measurements of the xylem pressure of Nicotiana plants by means of the pressure bomb and pressure probe[J].Planta, 1990, 182(3):325-338.

[6] Zhu J J,Zimmermann U,Thurmer F,Haase A.Xylem pressure response in maize roots subjected to osmotic stress:[JP2]determination of radial reflection coefficients by use of the xylem pressure probe[J].Plant Cell Environ, 1995, 18(8):906-912.

[7] Cochard H,Ewers F W,Tyree M T.Water relations of a tropical vine-like bamboo (Rhipidocladum racemiflorum):root pressures,vulnerability to cavitation and seasonal changes in embolism[J].J Exp Bot, 1994, 45(8):1085-1089.

[8] Zimmermann U,Schneider H,Wegner L H,Wagner H J,Szimtenings M,Haase A,Bentrup F W.What are the driving forces for water lifting in the xylem conduit?[J]Physiol Plantarum, 2002, 114(3):327-335.

[9] Schneider H,Zhu J J,Zimmermann U.Xylem and cell turgor pressure probe measurements in intact roots of glycophytes:transpiration induces a change in the radial and cellular reflection coefficients[J].Plant Cell Environ, 1997, 20(2):221-229.

[10] ThÜrmer F,Zhu J J,Gierlinger N,Schneider H,Benkert R,Gener P,Herrmann B,Bentrup F W,Zimmerniann U.Diurnal changes in xylem pressure and mesophyll cell turgor pressure of the liana Tetrastigma voinicrianum:The role of cell turgor in long-distance water transport[J].Protoplasma, 1999, 206(1-3):152-162.

[11] 刘小芳,张岁岐,杨晓青.压力探针技术原理及其在植物水分关系研究中的应用[J].干旱地区农业研究, 2008, 26(4):172-179.

[12] 万贤崇,张岁岐,张文浩.压力探针技术在植物水分关系研究中的运用[J].植物生理与分子生物学学报,2007,33(6):471-479.

[13] 万贤崇,叶清.植物生理学研究中的压力探针技术[J].植物学通报, 2008, 25(4):497-506.

[14] 朱建军,柏新富,张萍,卜庆梅.盐胁迫下大麦根系木质部压力的自调节现象[J].植物生理与分子生物学学报, 2005, 31(1):97-102.

[15] 柏新富,朱建军,卜庆梅,蒋小满.盐胁迫对大豆根系木质部压力和Na+吸收的影响[J].生态学报, 2009, 29(12):6506-6511.

[16] Zhu J J,Bai X F,Bu Q M,Jiang X M.An analysis to the driving forces for water and salt absorption in roots of maize seedlings under salt stress[J].Agric Sci China, 2010, 9(6):806-812.