DOI: 10.3724/SP.J.1206.2011.00132

Progress in Biochemistry and Biophysics (生物化学与生物物理进展) 2011/38:10 PP.929-935

Characterization of Epitope Peptides of Neuraminidases of The 2009 A H1N1 Viruses Designed by Immunoinformatics*

The neuraminidase (NA) of influenza virus is a receptor-destroying enzyme, removing sialic acid from carbohydrate chains attached to HA, and releasing the viruses from infected cells. The NA genes of the 2009 A H1N1 were sequenced, then the B-cell epitopes were predicted, screened and assessed based on immunoinformatics. Two peptides SE8 and RE6 (amino acid residues 168~175 and 428~433) of NA protein were synthesized and immunized to raise antisera in rabbits. Two antisera are capable of eliciting neutralizing antibodies against 2009 A H1N1 in the in vitro microneutralization assay, furthermore, the anti-releasing effects of hemagglutination existed in the antisera. Alignment with databases showed that the amino acid residues of two epitope peptides are highly conserved amongst the NA sequences of the strains isolated from the world. These findings indicate that SE8 and RE6 represents an attractive candidate for an effective synthetic peptide-based vaccine against 2009 A H1N1 viruses.

Key words:2009 A H1N1, neuraminidase (NA), B-cell epitope, immunoinformatics

ReleaseDate:2014-07-21 15:58:06

Funds:This work was supported by a grant from The National Natural Science Foundation of China (30972757)

[1] Trifonov V, Khiabanian H, Rabadan R. Geographic dependence, surveillance, and origins of the 2009 influenza A (H1N1) virus. N Engl J Med, 2009, 361(2): 115-119

[2] Solovyov A, Palacios G, Briese T, et al. Cluster analysis of the origins of the new influenza A(H1N1) virus. Eurosurveill, 2009, 14(21) pii: 19224

[3] Clark T W, Pareek M, Hoschler K, et al. Trial of 2009 Influenza A (H1N1) Monovalent MF59-Adjuvanted Vaccine. N Engl J Med, 2009, 361(25): 2424-2435

[4] Huang P, Yu S Y, Ke C W. Stepwise prediction and statistical screening:the B-cell epitopes on neuraminidase of human avian H5N1 virus. Chin Sci Bull, 2008, 53(23): 3642-3647

[5] Huang P, Xu Y, Ni H, et al. Linear B-cell epitope mapping of neuraminidases of the 2009 A H1N1 viruses based on immunoinformatics. Vaccine, 2011, 29(6): 1278-1282

[6] Arnold K, Bordoli L, Kopp J, et al. The SWISS-MODEL Workspace: A web-based environment for protein structure homology modeling. Bioinformatics, 2006, 22(2):195-201

[7] Krieger E, Koraimann G, Vriend G. Increasing the precision of comparative models with YASARA NOVA-a self-parameterizing force field. Proteins, 2002, 47(3): 393-402

[8] Zhao X L, Yin J, Chen W Q, et al. Generation and characterization of human monoclonal antibodies to G5, a linear neutralization epitope on glycoprotein of rabies virus, by phage display technology. Microbiol Immunol, 2008, 52(2): 89-93

[9] Uhlendorff J, Matrosovich T, Klenk H, et al. Matrosovich M. Functional significance of the hemadsorption activity of influenza virus neuraminidase and its alteration in pandemic viruses. Arch Virol, 2009, 154(6): 945-957

[10] Huang P, Deng F. Influenza. Guangzhou: Guangdong Technological Press, 2004: 89-91

[11] 郭慧芳, 张文红, 温冬青, 等. Ur eB蛋白B细胞抗原表位快速筛选与鉴定. 生物化学与生物物理进展, 2006, 33(1): 83-86 Guo H F, Zhang W H, Wen D Q, et al. Prog Biochem Biophys, 2006, 33(1): 83-86

[12] 王本旭, 刘 玉, 刘 展, 等. 应用噬菌体展示技术筛选人巨细胞病毒糖蛋白M新的中和抗原表位. 生物化学与生物物理进展, 2009, 36(2): 220-227 Wang B X, Liu Y, Liu Z, et al. Prog Biochem Biophys, 2009, 36(2): 220-227

[13] De Groot A S, Sbai H, Aubin C S, et al. Immunoinformatics: Mining genomes for vaccine components. Immunol Cell Biol, 2002, 80(3): 255-269

[14] Kamps B S, Hoffmann C, Preiser W. Influenza Report 2006. Paris: Flying Publisher, 2006: 54-56

[15] Rajik M, Omar A R, Ideris A, et al. A novel peptide inhibits the influenza virus replication by preventing the viral attachment to the host cells. Int J Biol Sci, 2009, 5(6): 543-548