DOI: 10.3724/SP.J.1206.2012.00597

Progress in Biochemistry and Biophysics (生物化学与生物物理进展) 2013/40:12 PP.1193-1200

Research Progress in Analyzing Transcriptome at The Level of Single Cells

Analysis of the transcriptomes of single cells can provide a comprehensive understanding of theheterogeneous expression and gene regulatory networks within a population of cells. Through mRNA isolation,reverse transcription, amplification and deep-sequencing analysis, the transcriptional landscape can be obtained forsubsequent detailed quantitative investigation of the levels and variation of gene expression underlying therelationship between the genotype and phenotype of a single cell. Not surprisingly, it has been applied within manyfields such as developmental biology, basic medicine, clinical research and drug discovery. This review introducesthe history, progress, and technological characteristics of whole transcriptome analyses of single cells, as well asdetailed strategies and advantages of the methodology. The technical challenges, potential applications, and futuredevelopments are also discussed.

Key words:single-cell,transcriptome,RNA-Seq

ReleaseDate:2015-04-18 09:14:49

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