doi:

DOI: 10.3724/SP.J.1206.2012.00620

Progress in Biochemistry and Biophysics (生物化学与生物物理进展) 2013/40:12 PP.1220-1229

BMP9 Regulates Osteogenic Differentiation of Mesenchymal Stem Cells Through JNKs Kinase Pathway


Abstract:
In addition to Smad pathway, our previous study has shown that BMP9 can induce osteogenicdifferentiation of mesenchymal stem cells (MSCs) through p38 MAPKs pathway. In this study, we explore thepossible involvement and detail role of JNKs (c-Jun N-terminal kinases) in BMP9-indcued osteogenicdifferentiation of MSCs. BMP9 was introduced into MSCs by recombinant adenoviruses protocol, then, in vivoand in vivo assays were conducted to detect whether BMP9 can induce osteogenic differentiation of MSCs throughJNKs kinase pathway. The results showed that BMP9 can activate JNKs kinase through increase thephosphorylated form of JNKs kinase. JNKs kinase inhibitor SP600125 can inhibit ALP activity, OPN and OCNexpression, as well as calcium deposition induced by BMP9 in MSCs. Furthermore, SP600125 also led to adecrease in BMP9-induced Runx2 activity and canonical Smad signaling. Moreover, when JNKs kinase wassilenced by RNA interference in MSCs, BMP9-induced osteogenic differentiation in vivo and ectopic boneformation in vivo were accordingly inhibited along with knockdown of JNKs. Taken together, those resultsintensively suggested that BMP9 can induce and regulate osteogenic differentiation of MSCs through activatingJNKs kinase pathway.

Key words:bone morphogenetic proteins 9,JNKs,mesenchymal stem cells,osteogenic differentiation,mitogen activated protein kinase

ReleaseDate:2015-04-18 09:14:50



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