DOI: 10.3724/SP.J.1141.2008.00049

Zoological Research (动物学研究) 2008/29:1 PP.49-55

Assessing Exploratory Behavior and Memory in ICR, BALB/c and C57BL/6 Mice Using Habituation

Differences in exploratory, rearing and locomotion activity of ICR, BALB/c and C57BL/6 strain were investigated by a 16-hole board task and an open field with an automated infrared detecting system. ICR mice had the highest response rate while C57BL/6 mice had the lowest. It took less time for ICR mice to get used to a new environment and its performance in memory testing was also superior to C57BL/6 mice. The results confirmed the feasibility of using a hole-board test to assess memory. Comparisons between the trend of rearing and hole-poking activity demonstrated that the rearing behavior of mice, to a certain degree, reflected its exploratory and cognitive ability. Our results provided firm support for the notion that selection of a mouse strain is essential when conducting studies on higher cognitive behavior in mice.

Key words:Animal behavior,Strain,Exploratory behavior,Habituation,Memory

ReleaseDate:2014-07-21 14:08:07

[1]Brodkin J. 1999. Assessing memory in mice using habituation of nose-poke responding[J]. Behav Pharmacol,10(5):445-51.

[2]Calamandrei G, Pennazza S, Ricceri L, Valanzano A. 1996. Neonatal exposure to anti-nerve growth factor antibodies affects exploratory behavior of developing mice in the hole board[J]. Neurotoxicol Teratol,18(2):141-6.

[3]Edsbagge J, Zhu S, Xiao MY, Wigström H, Mohammed AH, Semb H. 2004Expression of dominant negative cadherin in the adult mouse brain modifies rearing behavior[J]. Mol Cell Neurosci,25(3):524-35

[4]Fatemi SH, Pearce DA, Brooks AI, Sidwell RW. 2005. Prenatal viral infection in mouse causes differential expression of genes in brains of mouse progeny: A potential animal model for schizophrenia and autism[J]. Synapse,57(2):91-9.

[5]Kalueff AV, Wheaton M, Murphy DL. 2007. What's wrong with my mousemodel Advances and strategies in animal modeling of anxiety and depression[J]. Behav Brain Res,179(1):1-18

[6]Kamei J, Matsunawa Y, Miyata S, Tanaka S, Saitoh A. 2004. Effects of nociceptin on the exploratory behavior of mice in the hole-board test[J]. Eur J Pharmacol,489(1-2):77-87.

[7]Keeney AJ, Hogg S. 1999. Behavioural consequences of repeated social defeat in the mouse: preliminary evaluation of a potential animal model of depression[J]. Behav Pharmacol,10(8):753-64.

[8]Mastropaolo J, Rosse RB, Deutsch SI. 2004. Anabasine, a selective nicotinic acetylcholine receptor agonist, antagonizes MK-801-elicited mouse popping behavior, an animal model of schizophrenia[J]. Behav Brain Res,153(2):419-22.

[9]Pappas GD, Kriho V, Liu WS, Tremolizzo L, Lugli G, Larson J. 2003. Immunocytochemical localization of reelin in the olfactory bulb of the heterozygous reeler mouse: an animal model for schizophrenia[J]. Neurol Res,25(8):819-30.

[10]Platel A, Porsolt RD. 1982. Habituation of exploratory activity in mice: a screening test for memory enhancing drugs[J]. Psychopharmacology,78:346-52.

[11]Thompson RF, Spencer WA. 1966. Habituation: a model phenomenon for the study of neuronal substrates of behavior[J]. Psychol Rev,73:16-43.

[12]Ukai M, Kobayashi T, Kameyama. 1994. Effects of several amnesiac drugs on the habituation of exploratory activity in mice as indexed by horizontal and vertical activities[J]. Gen Pharmacol,25:179-85.