doi:

DOI: 10.3724/SP.J.1141.2013.02116

Zoological Research (动物学研究) 2013/34:2 PP.116-120

Isolation and identification of Tupaia orthoreovirus


Abstract:
Pathogenic viruses can harm acutely the life and health of laboratory tree shrews acutely; however, few papers exist regarding natural pathogenic virus infection in this species. Six fecal samples obtained from dead tree shrews were collected. The fecal supernatant infected Vero cell line resulted in cytopathic effects (CPE) after 72 h. The CPE included granulating, shrinking, rounding, seining and falling off. Electron microscopy showed the isolation was spherical, double-layered capsid, and about 75 nm in diameter. The purified isolation genome was 10 segments in a typical 3:3:4 arrangements, as shown by polyacrylamide gel electrophoresis (PAGE). The isolation was confirmed by RT-PCR assays targeting the conserved region of the L1 gene, sequence analysis and reconstruction of a phylogenetic tree. The isolation was a Tupaia Orthoreovirus (TRV), belonging to Mammalian Orthoreovirus (MRV). The obtained strain had the closest phylogenetic relationship to the MRV strain T3/Bat/Germany/342/08. As a zoonotic virus, the novel TRV strain was first isolated from wild tree shrews, which is significant for promoting tree shrew standardization and providing scientific data for preventing zoonotic tree shrew-to-human transmission.

Key words:Tree shrew: Tupaia reovirus,Isolation,Identification

ReleaseDate:2015-04-16 19:48:04



Attoui H, Biagini P, Stirling J, Mertens PP, Cantaloube JF, Meyer A, De Micco P, De Lamballerie X. 2001. Sequence characterization of Ndelle virus genome segments 1, 5, 7, 8, and 10: evidence for reassignment to the genus Orthoreovirus, family Reoviridae. Biochemical and Biophysical Research Communication, 287(2): 583-588.

Cashdollar LW, Chmelo RA, Wiener JR, Joklik WK. 1985. Sequences of the S1 genes of the three serotypes of reovirus. Proceedings of the National Academy of Sciences of the United States of America, 82(1): 24-28.

Chang JT, Li X, Zhang YL, Yu L. 2008. Isolation and identification of bovine reovirus from feces of the calves suffered from diarrhea. Chinese Journal of preventive Veterinary Medicine, 30(9):711-715. [常继涛, 李鑫, 张亚科, 于力. 2008. 犊牛腹泻粪样中牛呼肠孤病毒的分离鉴定. 中国预防兽医学报, 30(9): 711-715.]

Chua KB, Voon K, Yu M, Keniscope C, Rasid KA, Wang LF. 2011. Investigation of a potential zoonotic transmission of orthoreovirus associated with acute influenza-like illness in an adult patient. PLoS One, 6(10): e25434.

Debiasi RL, Edelstein CL, Sherry B, Tyler KL. 2001. Calpain inhibition protects against virus-induced apoptotic myocardia injury. Journal of Virology, 75(1): 351-361.

Huang ZX, Hong T, Sun CB. 1990. Medical Virology Basis, Fundamental Techniques and Methods. Beijing: Science Press, 608-612. [黄祯祥, 洪涛, 孙崇柏. 1990. 医学病毒学基础及实验技术. 北京: 科学出版社, 608-612. ]

Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE. 2001. Fields Virology. 4th ed. Philadelphia: Lippincott Willians and Wilkins, 2636-2663.

Koh C, Lesnik R, Brinkmann A, Ebinger A, Radoni? A, Nitsche A, Mühldorfer K, Wibbelt G, Kurth A. 2012. Isolation and characterization of three mammalian orthoroviruses from European Bats. PLoS One, 7(8): e43106.

Leary TP, Erker JC, Chalmers ML, Wetzel JD, Desai SM, Mushahwar IK, Dermody TS. 2002. Detection of reovirus by reverse transcription-polymerase chain reaction usingprimers corresponding to conserved regions of the viral L1genome segment. Journal of Virological Methods, 104(2): 161-165.

London L, Majeski EI, Paintlia MK, Harley RA, London SD. 2003. Respiratory reovirus 1/L induction of diffuse alveolar damage: a model of acute respiratory distress syndrome. Experimental and Molecular Pathology, 72(1): 24-36.

Majeski EI, Paintlia MK, Lopez AD, Harley RA, London SD, London L. 2003a. Respiratory reovirus 1/L induction of intraluminal fibrosis, a model of bronchiolitis obliterans organizing pneumonia, is dependent on T lymphocytes. American Journal of Pathology, 163(4): 1467-1479.

Majeski EI, Harley RA, Bellum SC, London SD, London L. 2003b. Differential role for T cells in the development of fibrotic lesions associated with reovirus 1/L-induced bronchiolitis obliterans organizing pneumonia versus Acute Respiratory Distress Syndrome. American Journal of Respiratory Cell and Molecular Biology, 28(2): 208-217.

McCrae MA, Joklik WK. 1978. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology, 89(2): 578-593.

Sabin AB. 1959. Reoviruses. Science, 130(3386): 1387-1389.

Spinner ML, Di Giovanni GD. 2001. Detection and identification of mammalian reoviruses in surface water by combined cell culture and reverse transcription-PCR. Applied and Environmental Microbiology, 67(7): 3016-3020.

Tyler KL, Sokol RJ, Oberhaus SM, Le M, Karrer FM, Narkewicz MR, Tyson RW, Murphy JR, Low R, Brown WR. 1998. Detection of reovirus RNA in hepatobiliary tissues from patients with extrahepatic biliary atresia and choledochal cysts. Hepatology, 27(6): 1475-1482.

Tyler KL, Barton ES, Ibach ML, Robinson C, Campbell JA, Donnell SM, Valyi-Nagy T, Clarke P, Wetzel JD, Dermody TS. 2004. Isolation and molecular characterization of a novel type 3 reovirus from a child with meningitis. Journal of Infectious Diseases, 189(9): 1664-1675.

Tillotson JR, Lemer AM. 1967. Reovirus type 3 associated with fatal pneumonia. The New England Journal of Medicine, 276(19): 1060-1063.

Van Regenmortel MHV, Fauquet CM, Bishop DHL. 2003. Virus Taxonomy Seventh Report of the International Committee on Taxonomy of Viruses. San Diego: Academic Press, 84-96.

Zeng ZY, Guo WZ, Xu ZW, Song ZH. 2007. Isolation, identification of porcine reovirus from diarrhea feces of pigs. Acta Veterinaria et Zootechnica Sinica, 38(6): 574-580. [曾智勇, 郭万柱, 徐志文, 宋振辉, 殷华平, 王新, 王小玉. 仔猪腹泻粪样中猪呼肠孤病毒的分离鉴定. 畜牧兽医学报, 38(6): 574-580.)

Zou TT, Tan H, He J, Zhu H, Zhang HJ, Song LH, Huang RT, Duan Q. 2003. Cloning and identification of reovirus isolated from specimens of SARS patients. Bulletin of the Academy of Military Medical Sciences, 27(4): 241-243. [左庭婷, 檀华, 何君, 朱虹, 张浩杰, 宋立华, 黄如统, 端青. 2003. 从SARS患者标本中分离的呼肠病毒的分离及鉴定. 军事医学科学院院刊, 27(4): 241-243. ]