DOI: 10.3724/SP.J.1141.2008.06585

Zoological Research (动物学研究) 2008/29:6 PP.585-591

Molecular Phylogeny of the Higher Category of Acrididae (Orthoptera: Acridoidea)

The phylogenetic relationships among all taxa within the Acrididae (Orthoptera: Acridoidea) were largely unknown until now. In this study, to further investigations, 24 species of Acrididae from China were used as sample taxa. The sequence constitutions and variations were analyzed and the molecular phylogenetic trees were reconstructed based on the combined sequence data (795 bp length in total) of 12S rDNA and 16S rDNA, using the grasshopper Pyrgomorpha conica of Pyrgomorphidae as the outgroup. The results showed that the rates of the two kinds of transitions are obviously much higher than that of the four kinds of transversions in these combined 12S+16S rDNA sequence data. The saturation of nucleotide substitutions happened in 12S and 16S rDNA sequence data. The molecular phylogenetic trees indicated that Oedipodinae is a monophyletic group and this subfamily is a natural one, but Catantopinae and Acridinae are non-monophyletic. Oedipodnae is a relatively primitive group within the Acrididae, whereas the Oxyinae may have diverged later than Oedipodinae, but earlier than most other species of Acridide.

Key words:Phylogeny,Acrididae,16S rDNA,12S rDNA

ReleaseDate:2014-07-21 14:49:14

Funds:Supported by National Natural Science Foundation of China (30160015)

Chen XF, Wang X, Yuan XD, Tang MQ, Li YX, Guo YM, Li QW. 2003. Sequence variation of mitochondrial cytochromebgene and phylogenetic relationships among twelve species of Charadriiformes[J].Acta Genetica Sinica, 30 (5): 419-4241.

Cognato AI, Sperling FAH. 2000. Phylogeny of Ips DeGeer species (Coleoptera: Scolytidae) inferred from mitochondrial cytochrome oxidase I DNA sequence[J].Molecular Phylogenetics and Evolution, 14(3): 445-460.

Desalle R, Freedman T, Prager EM. 1997. Tempo and mode of sequence evolution in mitochondrial DNA of Hawaiian Drosophila[J].J Mol Evol, 26:157-164.

Flook PK, Klee S, Rowell CHF. 1999. Combined molecular analysis of the Orthoptera and implications for their higher systematics[J].Syst Biol, 48(2): 233-253.

Flook PK, Rowell CHF. 1997. The Phylogeny of the Caelifera (Insecta, Orthoptera) as deduced from mtrRNA gene sequences[J].Mol Phylogenet Evol, 8(1): 89-103.

Huelsenbeck JP, Ronquist F. 2001. MRBAYES: Bayesian inference of phylogenetic trees[J].Bioinformatics, 17: 754-755.

Jiang GF, Zheng ZM. 1998. Grasshoppers and Locusts from Guangxi[M]. Guilin: Guangxi Normal University Press.

Kevan DKM. 1982. Orthoptera. In: Parker SP ed. Synopsis and Classification of Living Organisms[M]. New York: McGraw-Hill, 352-379.

Kirby, W. 1825. Some remarks on the nomenclature of the Gryllina of MacLeay, with the characters of a new genus in that tribe[J].Zoological Journal, 1: 429-432.

Knight A, Mindell DP. 1993. Subsititions bias, weighting of DNA sequence evolution, and the phylogenetic positions of Fea’s viper[J].Syst Biol, 42(1): 18-31.

Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0[J].Molecular Biology and Evolution, 24(8): 1596-1599.

Li HZ, Xia KL. 2006. Fauna Sinica, Insecta Vol. 43. Orthoptera: Acridoidea (Catantopidae) [M].Beijing: Science Press.

Liu DF, Jiang GF. 2005. Molecular phylogenetic analysis of Acridoidea based on 18S rDNA with a discussion on its taxonomic system[J].Acta Entomologica Sinica, 48 (2): 232-241.

Liu DF , Jiang GF,Shi H, Sun ZL, Huo GM. 2005. Monophyly and the taxonomic status of subfamilies of the Catantopidae based on 16S rDNA sequences[J].Acta Entomologica Sinica, 48(5): 759-769.

Lu HM, Huang Y. 2006. Phylogenetic relationship of 16 Oedipodidae species (Insecta: Orthoptera) based on the 16S rRNA gene sequences[J].Insect Science, 13(2): 103-108.

Rowell CHF, Flook PK. 1998. Phylogeny of the Caelifera and the Orthoptera as derived from ribosomal gene sequences[J].J Orthoptera Research, 7: 147-156.

Eades DC, Otte D, Naskrecki P. 2007. Orthoptera Species File Online. Version 2.0/3.1. .

Simon C, Frati F, Beckenbach A, Crespi B , Liu H , Flook P. 1994. Evolution , weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved PCR primers[J].Ann Entomol Soc Am, 87: 651-7011.

Sun ZL, Jiang GF, Huo GM, Liu DF. 2006. A phylogenetic analysis of six genera of Acrididae and the monophyly of Acrididae in China using 16S rDNA sequences (Orthoptera, Acridoidea) [J].Acta Zoologica Sinica, 52(2): 302-308.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research, 24: 4876-4882.

Walker F. 1871. Catalogue of the Specimens of Dermaptera Saltatoria in the Collection of the British Museum [M]. Supplement: 102.

Xia KL. 1994. Fauna Sinica, Insecta Vol.4. Orthoptera: Acridoidea (Pamphagidae, Chrotogonidae, Pyrgomorphidae) [M]. Beijing: Science Press.

Yin XC, Xia KL. 2003. Fauna Sinica, Insecta Vol.32, Gomphoceridae and Acridoidea[M]. Beijing: Science Press.

Zheng ZM. 1993. Taxonomy of Grasshoppers[M]. Xi’an: Shanxi Normal University Press.

Zheng ZM, Xia KL. 1998. Fauna Sinica, Insecta Vol. 10. Orthoptera: Acridoidea (Oedipodidae and Arcypteridae) [M]. Beijing: Science Press.