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Chinese Journal of Chromatography


DOI: 10.3724/SP.J.1123.2010.00089

Chinese Journal of Chromatography 2010/28 :1 PP.89-92

Technical Notes

Determination of α-arbutin, β-arbutin and niacinamide in cosmetics by high performance liquid chromatography

CHENG Peng 1 , CHEN Meilan 2 & ZHU Yan 1, 3

1. College of Chemistry and Biology Engineering, Guilin University of Technology, Guilin, 541004, China
2. College of Biology and Environment Engineering, Zhejiang Shuren University, Hangzhou, 310015, China
3. Department of Chemistry, College of Science, Zhejiang University, Hangzhou, 310028, China

Abstract: A high performance liquid chromatography (HPLC) method for the determination of two optical isomers of arbutin (α-arbutin and β-arbutin) and niacinamide in cosmetics was developed. The samples were extracted by the mixture of salt water and chloroform (2:1, v/v). The separation was performed on an ODS-BP column (200 mm×4.6 mm, 5 μm, Elite) with methanol-water (10:90, v/v) as the mobile phase at a flow rate of 0.5 mL/min and 25 ℃. The detection wavelength was set at 220 nm and the sample injection volume was 20 μL. There were good linear relationships between the mass concentration and the peak areas of α-arbutin, β-arbutin and niacinamide in the ranges of 0.07~50, 0.06~50 and 0.05~50 mg/L, respectively. The relative standard deviations (RSDs, n=7) of α-arbutin, β-arbutin and niacinamide were 1.65%, 1.73% and 1.33%, respectively. The proposed method has been applied for the determination of α-arbutin, β-arbutin and niacinamide in cosmetics with recoveries of 91.7%~109.6%. This method is rapid, simple and suitable for the detection of whitening ingredients in cosmetic.

Keywords: high performance liquid chromatography (HPLC)  α-arbutin  β-arbutin  niacinamide  cosmetics 

Recieved: 2009-05-14 ; Accepted: 2010-03-15; Published Online: 2010-03-15

Corresponding Author: ZHU Yan (zhuyan@zju.edu.cn)