doi:

DOI: 10.3724/SP.J.1145.2011.00069

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2011/17:1 PP.69-72

Effects of Shrimp Aquaculture on the Quality of Upland Pond Soil Indicating by Microbial FAME and Enzyme Activity*


Abstract:
With the rapid expanding of shrimp aquaculture, upland ponds have been built extensively along the sea coast in southern China. In order to evaluate the influence of shrimp aquaculture on soil quality, soil samples were collected from a typical site, Baimajing of the Hainan Island. Analyses of fatty acid methyl ester (FAME) and soil enzyme activities were done to monitor and compare the changes in soil microbial community compositions and activities. The pH and salinity of upland pond soil were significantly higher than control, but organic matter and total N showed adverse pattern (P<0.05). The fatty acid of bacteria, fungi and actinobacteria declined significantly in upland pond soil, even the fungi decreased sharply (P<0.05) and actinobacteria could not be detected. The three enzymatic activities (dehydrogenase, urease, and acid phosphatase) also decreased significantly (P<0.05). These results showed that the soil would be degraded after long-term shrimp aquaculture. Fig 2, Tab 4, Ref 29

Key words:upland pond,FAME analysis,soil enzyme,soil microbe,soil quality,Hainan Island

ReleaseDate:2014-07-21 15:53:52

Funds:Supported by the Key Projects of New Varieties of GMO Cultivation of China (No. 2009ZX08009-120B) and the Sci-tech Project of Huazhong Agricultural University (No. 52204-07028)



1 Wang L (王凌). Analysis on environmental economy and pattern of high position pool aquiculture. Environ Prot Sci (环境保护科学), 2008, 34 (4): 54~57

2 Xie DH (谢东海), Han Q (韩奇), Tang WH (唐文浩). Recent advance in the application research on ecological environmental effects of marine aquaculture. Jiangsu Environ Sci & Technol (江苏环境科技), 2005, 18 (z1): 10~12

3 Towatana P, Voradaj C, Panapitukkul N. Changes in soil properties of abandoned shrimp ponds in southern Thailand. Environ Monit Assess, 2002, 74: 45~65

4 Gil-Sotres F, Trasar-Cepeda C, Leirós MC, Seoane S. Different approaches to evaluating soil quality using biochemical properties. Soil Biol Biochem, 2005, 37: 877~887

5 Huang CY (黄昌勇). 土壤学. Beijing, China: China Agriculture Press (北京: 中国农业出版社), 2000

6 Islam RM, Trivedi P, Palaniappan P, Reddy MS, Sa T. Evaluating the effect of fertilizer application on soil microbial community structure in rice based cropping system using fatty acid methyl esters (FAME) analysis. World J Microbiol Biotechnol, 2009, 25: 1115~1117

7 Ryan J, Estefan G, Rashid A. Soil and Plant Qnalysis Laboratory Manual. Aleppo, Syria: International Center for Agricultural Research in the Dry Area (ICARDA), 2001. 46~102

8 Cai YF (蔡燕飞), Liao ZW (廖宗文). Advancement of methods in soil microbial ecology. Soil & Environ Sci (土壤与环境), 2002, 11 (2): 167~171

9 Frostegåd Å, Bååth E. The use of phospholipid fatty acid to estimate bacterial and fungal biomass in soil. Biol Fert Soils, 1996, 22: 59~65

10 Zogg GP, Zak DR, Ringleberg DB, MacDonald NW, Pregitzer KS, White DC. Compositional andfunctional shifts inmicrobial communities due to soil warming. Soil Sci Soc Am J, 1997, 61: 475~481

11 Jennifer MK, Dick RP. PLFA profiling of microbial community structure and seasonal shifts in soils of a douglas-fir chronosequence. Microb Ecol, 2008, 55: 500~511

12 Marschner P. Soil microbial community structure and function assessed by FAME, PLFA and DGGE–advantages and limitations. Soil Biol, 2007, 11: 181~200

13 Hu ZB (胡子斌). Normal temperature extraction for the determination of TTC- dehydrogenase activity and its application. Ind Water Treatment (工业水处理), 2001, 21 (10): 29~31

14 Feng R (丰骁), Duan JP (段建平), Pu XP (蒲小鹏), Zou Y (邹雨), Li CR (李春荣). Comparative analyses between two methods of measuring soil urease activity. Grassl & Turf (草原与草坪), 2008, 127 (2): 70~72

15 Guan SY (关松荫). 土壤酶及其研究方法. Beijing, China: China Agriculture Press (北京: 中国农业出版社), 1986

16 Li FY(李福燕), Li XM (李许明), Wu PF (吴鹏飞), Chen LY (陈柳燕), Guo B (郭彬), Qi ZP (漆智平). Correlation between heavy metal pollution and basic properties of agricultural soils in hainan province. Soils (土壤), 2009, 41 (1): 49~53

17 Huang SJ (黄顺坚), Wu QZ (吴琼泽), Lv LW (吕烈武), Fu Z (符珠). The soil nutrients and measures of utilization of farmland in qionghai city of hainan province. Chin Agric Sci Bull (热带农业科学), 2006, 22 (5): 436~438

18 Guo ZH (郭朝晖), Liao BH (廖柏寒), HuangCY (黄昌勇). Microbial biomass carbon and enzyme activities in the tested soil treated with Cd, Cu and Zn by simulated acid rain. Chin J Appl Environ Biol (应用与环境生物学报), 2003, 9 (4): 382~385

19 Núñez-Regueira L, Rodríguez-Añón JA, Proupín-Castiñeiras J, Villanueva-López M, Núñez-Fernández O. Study of the influence of different forest species on the microbial activity in soils. J Therm Anal Calorim, 2006, 84 (1): 7~13

20 Yuan BC, Li ZZ, Liu H, Gao M, Zhang YY. Microbial biomass and activity in salt affected soils under arid conditions. Appl Soil Ecol, 2007, 35: 319~328

21 Sardinha M, Muller T, Schmeisky H, Joergensen RG. Microbial performance in soils along a salinity gradient under acidic conditions. Appl Soil Ecol, 2003, 23: 237~244

22 Tripathi S, Kumari S, Chakraborty A, Gupta A, Chakrabarti K, Bandyapadhyay B. Microbial biomass and its activities in salt-affected coastal soils. Biol Fertil Soils, 2006, 42: 273~277

23 Pankhurst CE, Yu S, Hawke BG, Harch BD. Capacity of fatty acid profiles and substrate utilization patters to describe differences in soil microbial communities associated with increased salinity or alkalinity at three locations in South Australia. Biol Fertil Soils, 2001, 33: 204~217

24 Avidano L, Gamalero E, Cossa GP, Carraro E. Characterization of soil health in an Italian polluted site by using microorganisms as bioindicators. Appl Soil Ecol, 2005, 30: 21~33

25 Madejón E, Moreno F, Murillo JM, Pelegrin F. Soil biochemical response to long-term conservation tillage under semi-arid Mediterranean conditions. Soil Till Res, 2007, 94: 346~352

26 Robertson GP, Groffman PM. Nitrogen transformations. In: Paul EA ed. Soil Microbiology, Ecology, and Biochemistry. USA: Elsevier, 2007. 341~364

27 Tabatabai MA. Soil enzymes. In: Weaver RW, Angle JS, Bottomley PS eds. Methods of Soil Analysis, Part 2, Microbiological and Biochemical Properties. Madison, WI, USA: Soil Sci Soc Am J, 1994. 775~833

28 Tripathi S, Chakraborty A, Chakrabarti K, Bandyopadhyay BK. Enzyme activities and microbial biomass in coastal soils of India. Soil Biol Biochem, 2007, 39: 2840~2848

29 Vries FT, Hoffland E, Eekeren N, Brussaard L, Bloem J. Fungal/bacterial ratios in grasslands with contrasting nitrogen management. Soil Biol Biochem 2006, 38: 2092~2103

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