DOI: 10.3724/SP.J.1249.2017.01027

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2017/34:1 PP.27-32

RNA-seq analysis on Arabidopsis ago1-27 mutant

miRNAs play important roles in plant development and adaptation to environment. We conduct the RNA-seq of Arabidopsis ago1-27 mutant and has identified thousands of differentially expressed genes. The study shows that miRNA targets tend to be upregulated in the ago1-27 mutant. New miRNA targets are identified by ago1-27 RNA-seq combined with degradome analysis. For examples, miR396 targets cysteine proteinase genes and miR167 targets a gene encoding AT-hook DNA-binding protein.

Key words:phytobiochemistry,microribonucleic acid (miRNA),ago1-27,RNA-seq,degradome,target genes,Arabidopsis

ReleaseDate:2017-01-17 15:39:43

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