doi:

DOI: 10.3724/SP.J.1249.2018.05529

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:5 PP.529-535

Two new molecular biomarkers in human colorectal cancer


Abstract:
In order to find new molecular biomarkers for early diagnose of colorectal cancer (CRC), we investigated the potential role of TATA-binding protein factor associated 1 like (TAF1L) and stomatin-like protein 1 (STOML1) in colorectal cancers and matched normal adjacent tissues by using tissue microarray with multiple immunohistochemical staining. Moreover, the relationship between TAF1L expressive level and deteriorated stages of CRC was also analyzed. The results showed that protein expression of TAF1L was significantly higher in CRC tissues than that in normal adjacent tissues (p<0.0001), and abnormal higher levels of TAF1L were significantly associated with the deterioration of CRC. In contrast, protein expression of STOML1 showed no statistical difference between colorectal cancer and normal adjacent tissue (p>0.05). The conclusion suggests that TAF1L overexpression is probably related to the occurrence and development of colorectal cancer, and may also be as a potential biomarker for early diagnosis, precision medicine and prognosis of colorectal cancer.

Key words:colorectal cancer,tissue microarray,multiple immunohistochemistry,TATA-box binding protein associated factor 1 like (TAF1L),stomatin-like protein 1(STOML1),biomarker

ReleaseDate:2018-12-14 06:52:41



[1] HARALDSDOTTIR S, EINARSDOTTIR H M, SMARADOTTIR A, et al. Colorectal cancer-review[J]. Laeknabladid,2014,100(2):75-82.

[2] ZHENG Zhaoxü, ZHENG Rongshou, ZHANG Siwei, et al. Colorectal cancer incidence and mortality in China, 2010[J]. Asian Pacific Journal of Cancer Prevention, 2014,15(19):8455-8460.

[3] CHEN Wanqing.Cancer statistics:updated cancer burden in China[J]. Chinese Journal of Cancer Research, 2015, 27(1):1.

[4] MILLER G, BIERNACKI P, KEMENY N E, et al. Outcomes after resection of synchronous or metachronous hepatic and pulmonary colorectal metastases[J].Journal of the American College of Surgeons,2007,205(2):231-236.

[5] PAIX A, ANTONI D, ADEDUNTAN R, et al. Stereotactic radiation therapy of brain metastases from colorectal cancer:a single institution cohort[J]. Cancer Radiother, 2017,21(3):199-204.

[6] OH H R, AN C H, YOO N J, et al. Frameshift mutations in the mononucleotide repeats of TAF1 and TAF1L genes in gastric and colorectal cancers with regional heterogeneity[J]. Pathology & Oncology Research,2017,23(1):125-130.

[7] WANG P J, PAGE D C. Functional substitution for TAF(Ⅱ)250 by a retroposed homolog that is expressed in human spermatogenesis[J]. Human Molecular Genetics,2002,11(19):2341-2346.

[8] LOUDER R K, HE Y, LOPEZ-BLANCE J R, et al.Structure of promoter-bound TFⅡD and model of human pre-initiation complex assembly[J]. Nature,2016:531(7596):604-609.

[9] BOUCHÉ L, CHRIST C D, SIEGEL S, et al.Benzoisoquinolinediones as potent and selective inhibitors of BRPF2 and TAF1/TAF1L bromodomains[J]. Journal of Medicinal Chemistry,2017,60(9):4002-4022.

[10] NAKAGAKI T, TAMURA M, KOBASHI K,et al.Profiling cancer-related gene mutations in oral squamous cell carcinoma from Japanese patients by targeted amplicon sequencing[J]. Oncotarget, 2017, 8(35):59113-59122.

[11] LAPATSINA L, BRAND J, POOLE K, et al. Stomatin-domain proteins[J]. European Journal of Cell Biology,2012,91(4):240-245.

[12] KOZLENKOV A, LAPATSINA L, LEWIN G R,et al. Subunit-specific inhibition of acid sensing ion channels by stomatin-like protein 1[J].The Journal of Physiology,2014,592(4):557-569.

[13] ZHANG Wei, MACDONALD E M, KOEPP D M. The stomatin-like protein SLP-1 and Cdk2 interact with the F-Box protein Fbw7-γ[J]. PLoS One, 2012, 7(10):e47736.

[14] ZHANG Qu, ZHANG Jun, JIN Hong,et al.Whole transcriptome sequencing identifies tumor-specific mutations in human oral squamous cell carcinoma[J]. BMC Medical Genomics, 2013, 6(1):28.

[15] 许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996, 6(4):229-231. XU Liangzhong, YANG Wentao. Standerds of immunohistochemistry result[J].China Oncology, 1996,6(4):229-231.(in Chinese)

[16] 杨军,康安静,苏宝山,等.免疫组织化学检测结果判读进展[J].中华临床医师杂志(电子版),2014, 8(20):3699-3703. YANG Jun, KANG Anjing, SU Baoshan,et al.Progress of scoring scheme of immunohistochemistry results[J].Chinese Journal of Clinicians (Electronic Edition),2014,8(20):3699-3703.(in Chinese)

[17] LAN Qiusheng, LAI Wei, ZENG Yujie,et al.CCL26 participates in the PRL-3-Induced promotion of colorectal cancer invasion by stimulating Tumor-Associated macrophage infiltration[J]. Molecular Cancer Therapeutics,2018,17(1):276-289.

[18] XIA Junfeng, JIA Peilin, HUTCHINSON K E,et al.A meta-analysis of somatic mutations from next generation sequencing of 241 melanomas:a road map for the study of genes with potential clinical relevance[J].Molecular Cancer Therapeutics,2014,13(7):1918-1928.

[19] WU Yong, LIN J C, PILUSO L G,et al.Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response[J].Molecular Cell,2014,53(1):63-74.

[20] LEE D H, GERSHENZON N, GUPTA M,et al.Functional characterization of core promoter elements:the downstream core element is recognized by TAF1[J].Molecular and Cellular Biology, 2005, 25(21):9674-9686.

[21] JUVEN-GERSHON T, KADONAGA J T.Regulation of gene expression via the core promoter and the basal transcriptional machinery[J].Developmental Biology, 2010,339(2):225-229.

[22] KIMURA J, NGUYEN S T, LIU Hanshao,et al.A functional genome-wide RNAi screen identifies TAF1 as a regulator for apoptosis in response to genotoxic stress[J].Nucleic Acids Research,2008,36(16):5250-5259.