doi:

DOI: 10.3724/SP.J.1249.2018.05536

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2018/35:5 PP.536-542

Nucleotidyl transferase proteins and the biological responses of Zea mays to abiotic stress


Abstract:
Nucleotidyl transferase proteins (NTPs) can modify the 3' ends of small RNA and mRNA and affect their stability. By analyzing the relationship between the domain of NTPs in Zea mays and the evolution of NTPs in Arabidopisis thaliana and Oryza sativa, 24 NTPs in Zea mays are identified, and the NTPs in Zea mays, Arabidopsis thaliana, and Oryza sativa are highly homologous and conserved. Sequence analysis shows that cis-acting elements of Zea mays NTP gene promoters contain many prmoter elements related to abiotic stress responses. Further analysis of real-time fluorescence quantitative polymerase chain reaction (RT-PCR) data shows that the expressions of NTP genes in Zea mays are induced by high salinity, drought, and abscisic acid stress, which indicates that NTPs may be involved in plant stress resistance pathway.

Key words:nucleotidyl transferase protein,Zea mays,Arabidopisis thaliana,Oryza sativa,cis-acting elements,realtime fluorescence quantitative polymerase chain reaction (RT-PCR),abiotic stress

ReleaseDate:2018-12-14 06:52:41



[1] ROGERS K, CHEN Xuemei.Biogenesis, Turnover, and mode of action of plant microRNAs[J]. Plant Cell, 2013, 25(7):2383-2399.

[2] ZHAO Yuanyuan, MO Beixin, CHEN Xuemei. Mechanisms that impact microRNA stability in plants[J]. RNA Biology, 2012, 9(10):1218-1223.

[3] SONG Jianbo, SONG Jun, MO Beixin, et al. Uridylation and adenylation of RNAs[J]. Science China Life Sciences, 2015, 58(11):1057-1066.

[4] HEO I, JOO C, KIM Y K, et al. TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation[J]. Cell, 2009, 138(4):696-708.

[5] RISSLAND O S, MIKULASOVA A, and NORBURY C J. Efficient RNA polyuridylation by noncanonical poly(A) polymerases[J]. Molecular and Cellular Biology, 2007, 27(10):3612-3624.

[6] IBRAHIM F, RYMARQUIS L A, KIM E J, et al. Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyl transferase promotes their degradation in Chlamydomonas[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(8):3906-3911.

[7] BORTOLAMIOL-BECET D, HU Fuqu, JEE D, et al. Selective suppression of the splicin g-mediated microRNA pathway by the terminal uridyltransferase Tailor[J]. Molecular Cell, 2015, 59(2):217-228.

[8] VAN WOLFSWINKEL J C, CLAYCOMB J M, BATISTA P J, et al. CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs[J]. Cell, 2009, 139(1):135-148.

[9] ZHAO Yuanyuan, YU Yu, ZHAI Jixian, et al. The arabidopsis nucleotidyl transferase HESO1 uridylates unmethylated small RNAs to trigger their degradation[J]. Current Biology:CB, 2012, 22(8):689-694.

[10] REN Guodong, CHEN Xuemei, YU Bin.Uridylation of miRNAs by HEN1 SUPPRESSOR1 in Arabidopsis[J]. Current Biology, 2012, 22(8):695-700.

[11] SEMENT F M, FERRIER E, ZUBER H, et al. Uridylation prevents 3' trimming of oligoadenylated mRNAs[J]. Nucleic Acids Research, 2013, 41(14):7115-7127.

[12] WANG Xiaoyan, ZHANG Shuxin, DOU Yongchao, et al. Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis[J]. PLoS Genetics, 2015, 11(4):e1005091.

[13] ZUBER H, SCHEER H, FERRIER E, et al. Uridylation and PABP cooperate to repair mRNA deadenylated ends in Arabidopsis[J]. Cell Reports, 2016, 14(11):2707-2717.

[14] TU Bin, LIU Li, XU Chi, et al. Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis[J]. PloS Genetics, 2015, 11(4):e1005119.

[15] SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Molecular responses to dehydration and low temperature:differences and cross-talk between two stress signaling pathways[J]. Current Opinion in Plant Biology, 2000, 3(3):217.

[16] PANTUWAN G, FUKAI S, COOPER M, et al. Yield response of rice (Oryza sativa L.) genotypes to different types of drought under rainfed lowlands:Part1. Grain yield and yield components[J]. Field Crops Research, 2002, 73(2):153-168.

[17] HSU Y T, KAO C H. Role of abscisic acid in cadmium tolerance of rice (Oryza sativa L.) seedlings[J]. Plant Cell and Environment, 2003,26(6):867-874.

[18] YANG Haiqi, SONG Jianbo, YUE Luming, et al. Identification and expression profiling of Oryza sativa nucleotidyl transferase protein (NTP) genes under various stress conditions[J]. Gene, 2017, 628:93-102.